Hard-coated filter sets for higher transmission efficiencies High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals. By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. In addition, new LED light and filter combinations maximize the signal-to-background ratio so critical for dim samples, improving data quality irrespective of signal strength for both EVOS and Countess systems. A larger aperture increases illumination uniformity, which affords superb tile-stitched image quality and consistency. Our second-generation light cube design dramatically improves on the original. Stable illumination intensity over 50,000 h helps experimental reproducibility.A short light path that affords superior detection of fluorescent signals.Optimized spectral compatibility to maximize true signal detection, while minimizing bleed-through for the best possible data quality.Uniform illumination across the entire field of view for superb image quality.The EVOS Light Cube, RFP 2.0, offers you these important advantages: The EVOS Light Cube, RFP 2.0, is ideal for use with Alexa Fluor 555, C圓, MitoTracker Orange CMTMRos, pHrodo, RFP, Rhod-2, and other fluorophores with excitation and emission maxima near 542 and 593 nm. Like the original light cubes, these LED cubes are user-changeable and automatically recognized by the instrument. These LED light cubes are backwards compatible with older EVOS and Countess systems and can be used with first-generation light cubes. Like the original design, they combine condensing optics and hard-coated filters into one remarkable light engine that delivers digitally controlled fluorophore excitation. At the same time, optimized spectral fidelity across channels significantly improves data quality by minimizing undesired bleed-through. Our second-generation EVOS LED light cubes provide superior illumination uniformity across the field of view, resulting in exceptional tile-stitch image quality.
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